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Protocol Directory

A list of protocols are provided below, sorted into application areas.  You can also use the search tool below to find protocols.

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Protocols

Animal Tissue Homogenization:

• Determination of a Nucleotide Diphosphate in Monkey and Rabbit Tissues
• DNA Extraction from Eois Moths
• DNA Extraction from Liver Tissue
• Drug extraction from brain and muscle rat tissues
• Drug Extraction from Whole Brain (Rat)
• Drug extraction from Whole Iris-Ciliary Body Pork
• Enzyme Assay from Frozen Abdominal Krill Tissue
• Homogenization of Aortic Tissue
• Homogenization of Atlantic Salmon (S. Salar) Muscle for Enzyme Activity Measurement
• Homogenization of C. elegans
• Homogenization of Cardiac Tissue
• Homogenization of Drosophila Adults
• Homogenization of Drosophila Larvae
• Homogenization of Drosophila for lipid analysis
• Homogenization of Fish Liver for Metabolomic & Transcriptomic Analysis
• Homogenization of Gill Tissue with Precellys Evolution
• Homogenization of Kidney Tissue
• Homogenization of Liver Tissue
• Homogenization of Mouse (Mus musculus) Artery for RNA Extraction
• Homogenization Protocol for Shellfish
• Homogenization of Tissue Maintaining Viable Bacteria
• Homogenization of Uterine Tissue
• Homogenization of Whole Rat (R. Norvegicus) Lung
• Homogenization of Zebrafish
• Improving Compound Extraction Efficiency
• Improving RNA Extraction from Mouse Tissues
• Isolation and Identification of Viable Immune Cells from Murine Lungs
• Bacterial Load within Infected Nematodes
• LPO Extraction from Tumor and Non-Tumor Rat tissues
• Metabolomic study from clam gill
• MicroRNA Expression Profiling of Individual Rat Hypothalamic Nuclei
• MicroRNA and TotalRNA Purification from Liver Tissue
• Mouse UCP-I Quantification in Brown Adipose Tissue
• Mouse Femur Homogenization in the Bullet Blender
• Prion Protein Extraction in Animal Tissues
• Protein extraction from mouse heart using the Minilys
• Protein extraction from mouse soft tissues
• Recover Live Herpes Virus from Infected Mouse Ganglia
• RNA Extractions of from Breast Cancer Xenografts and Lymph Node Metastases
• RNA Extraction from Caenorhabditis
• RNA Extraction from Human Colon
• RNA Extraction from Human Skin Biopsies
• RNA Extraction from Mouse Embryonic Tooth Germs
• RNA Extraction from Mouse Tissues
• RNA Extraction from Subcutaneous Adipose Tissue (scAT)
• RNA Extraction from Torafugu Pufferfish
• RNA Isolation from Rat Liver
• Total Protein Extraction
• Total RNA Extraction from Cartilage Tumors
• Viral RNA Extraction from Infected Tissues
• Viral RNA Titration in Chikungunya Inoculated Tissue Samples
• Whole Bacteria Isolation from Kidney, Lung, or Spleen

 

Plant Tissue Homogenization:

• DNA Extraction from Plant Leaves
• DNA Extraction from Rice Grain (Orysa sativa) using a Precellys 24
• DNA Extraction from Rice Leaf (Orysa sativa) using a Precellys 24
• Ginger Rhizome Homogenization in the Bullet Blender
• Homogenization of Arabidopsis thaliana
• Homogenization of Blueberries
• Homogenization of Corn / Maize Kernels
• Homogenization of Leek Leaf
• Homogenization of Malanga Root Tuber
• Homogenization of Peanut
• Homogenization of Pine Nut
• Homogenization of Sugar Cane
• Homogenization of Soybean
• Horseradish Root Homogenization in the Bullet Blender
• Improving Seagrass Grinding for Nutrient Analysis
• Metabolomic Study of the Red Algae Chondrus crispus
• Phosphate and sulfate assay extraction from plant
• Protein Extraction from Arabidopsis with Minilys
• Protein extraction from cashew nuts using the Precellys® Evolution
• Protein Extraction from Marine Phytoplankton
• Protein Extraction from Nicotiana benthamiana Leaf
• RNA Extraction from Arabidopsis
• RNA Extraction from Mycotoxigenic filamentous Fungi
• RNA Extraction from Plant Leaf with Minilys
• Seed grinding for GMO analysis using the Precellys® Evolution

 

Animal / Plant Cell Culture Lysis:

• Lysis of Drosophila S2 Cells using a Bullet Blender
• Virus Isolation using the Minilys Personal Homogenizer

 

Microbe Lysis:

• DNA Extraction from Baculovirus Granules
• DNA Extraction from Fungal Organisms
• Homogenization of Candida albicans for Protein Extraction
• Homogenization of Saccharomyces Cultures
• Homogenization of Streptococcus for Enzymatic Activity Measurements
• Lysing Micro-Organisims with the VK05 15 mL Precellys Lysing Kit
• Mechanical disruption of mycobacterial cells
• Metabolites extraction from Staphylococcus aureus
• Protein Extraction from Candida albicans
• Protein extraction from yeast Pichia pastoris
• RNA Extractions of Microorganisms
• RNA Extraction from Streptomyces using a Precellys 24
• RNA Extraction from Cheese
• RNA Extraction from Cyanobacteria using the Precellys 24
• Total RNA Extraction from Rhizobium etli Pellets

 

Forensic Applications:

• DNA Human Identification from Forensic Bone Fragments
• DNA Extraction from Fetal and Neonatal Pathology Tissues
• Drug Analysis in Hair
• High-throughput Lipid Extraction for the Analysis of Human Brain Lipids
• Post-mortem Redistribution of Drugs in Rats

 

Soil / Environmental Sample Preparation:

• DNA Extraction from Microbes in Soil
• Environmental Biomarkers Analysis in Moss
• Protein Extraction from Cypress Pollen
• Micromilling poorly water-soluble pharmaceutical API
• Sample extraction for quantification of mold antigen