Metabolomic study from clam gill
Instrument: Precellys® 24 Dual
Sample: Clam gill tissue
Protocol:
- Precellys® kit: 03961-1-003 (1.4mm ceramic beads).
- Add ~100 mg (wet mass) of gill tissue.
- Add Extraction solvents: 4 ml/g (v/w) methanol and 0.85 ml (v/w) water.
- Run the Precellys at 6400 rpm, 2x10 sec, 30 sec break.
Results:
Metabolomics analysis: Extraction with methanol, chloroform and water to extract the polar metabolites, storage at -80°C, one dimensional 1H NMR spectroscopy (500 M), data analysis.
