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• Homogenization of Malanga Root Tuber

Homogenization of Malanga Root Tuber

Instrument: Bullet Blender

Sample:  Xanthosoma sagittifolium

Protocol:

1. OPTIONAL: Wash malanga 3x with ~1mL PBS to remove soil and other surface contaminants and debris.
2. Cut malanga into long, thin slices of 200mg or less and place each slice into a microcentrifuge tube.
3. Add a volume of beads equal to the mass of the malanga. NOTE: 100mg ≅ 100μ
4. Close the microcentrifuge tubes and place them into the Bullet Blender®. NOTE: There should be no buffer in the tubes at this point.
5. Set controls for SPEED 8 and TIME 4.
6. Remove the samples from the Bullet Blender. The malanga should be finely pulverized into a thick paste. If not, run for another three minutes at speed 10.
7. Add 2 volumes of buffer to the tube for every mass of sample (ex. for 100 mg malanga add 200mL buffer).
8. Close the microcentrifuge tubes and place them back into the Bullet Blender®.
9. Set controls for SPEED 8 and TIME 3 minutes. Press Start.
10. After the run, remove tubes from the instrument.
11. Visually inspect samples. If homogenization is unsatisfactory, run for another three minutes at speed 10.
12. Proceed with your downstream application.

Results: