Instrument: Minilys Personal Homogenizer
Sample: Coprinopsis cinerea and Talaromyces emersonii
DNA extractions were performed on both T. emersonii and C. cinerea mycelia harvested from liquid culture. The combination of the Minilys® personal homogenizer with the E.N.Z.A fungal DNA extraction kit resulted in a successful and rapid DNA
extraction from both organisms. In order to increase efficiency for high throughput screening, the method was then tested on mycelia scraped from solid state cultures. DNA was successfully extracted from both organisms thus eliminating the need for liquid cultivation.
Figure 1: DNA extraction from plate mycelia performed using Minilys® bead mill, Precellys® lysing kit tubes and E.N.Z.A. fungal extraction kit on Coprinopsis cinerea FA2222. 1% agarose gel with gel red, lane 1: C. cinerea DNA, lane 2&3: 1kb molecular weight ladder.
Institute of Technology, Sligo, Ireland