DNA Extraction from Microbes in Soil
Instrument: Precellys® 24 Dual
Sample: Unadulterated soil
Protocol:
- Add 1g soil into tubes from 7ml soil grinding kits.
- Add 4ml buffer to each sample.
- Run the Precellys at 5000rpm, 3 x 15s.
Results:
DNA concentration was determined by electrophoresis and qPCR. DNA concentration using the Precellys was determined to be 2.5x higher than the legacy method (Mikro-Dismembrator S). Additionally, the Precellys provided a shorter run time and 6x more throughput.
Gel electrophoresis analysis from a soil sample (Epoisses) ran in triplicate. "Standard method" is the Mikro-Dismembrator S.
DNA concentration for three soil samples.
