Isolation of RNA from Rat Liver
Instrument: Precellys® 24 Dual
Sample: Mouse murine pancreas (organs in which the virus replicates)
Protocol:
- Precellys kits: CK28 (big ceramic beads)
- Sample : 30 mg of rat liver previously stored in RNAlater (Ambion) at -20°C
- Buffer : 600μl RTL buffer (RNeasy Mini Kit,Qiagen)
- Run the Precellys®24 at 5000rpm, 2x10 sec., 10 sec. break
Results:
These results were concluded with the collaboration of Altana Pharma AG. Analysis of the subsequently isolated RNA using the Agilent 2100 bioanalyzer.
The Precellys®24 is an ideal tool for the lysis of rat liver for subsequently isolating RNA. Particularly for higher numbers the Precellys® is superior to the formerly used mechanical blender, regarding time consumption and reproducibility.
