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• RNA Extractions of from Breast Cancer Xenografts and Lymph Node Metast

RNA Extractions of from Breast Cancer Xenografts and Lymph Node Metast

Instrument: Precellys® 24 Dual

Sample:  Tumors from breast cancer xenographs and lymph node metastases

Protocol:

• Precellys kits: CK14 (small ceramic beads)
• Sample : tumors from breast cancer xenographs and lymph node metastases (frozen)
• Buffer : 600μl of lysis buffer (RLT and ßmercaptoethanol)
• Run the Precellys®24 at 5500rpm, 1x20 sec.
• RNA extraction are performed following 10 different protocols (see below)

 

Results:

The Precellys® kits allow a quick and effective homogenization of the xenograft tissues, and the total RNA extracted with an appropriate kit following tissue lysis with the Precellys® kits is of good quality.

 

Gel electrophoresis analysis after the 10 protocols. Efficiency is validated on the primary tumors and lymph node metastases.