Homogenization and DNA Extraction from Rice Leaf (Orysa sativa)
Instrument: Precellys® 24
Sample: Rice (O. sativa) leaf
Protocol:
- Add rice leaf to 2ml tubes from the soil grinding kits.
- Briefly place tube in liquid nitrogen* to flash-freeze the sample.
- Run the Precellys 24 at 5000rpm, 1 x 15s.
- Briefly place tube in liquid nitrogen*
- Add DNA extraction buffer
- Pour the sample and buffer through a sieve to remove the beads from the sample
*In this instance, the DNA extraction buffer that was used required the sample to be frozen.
Results:
Left: Fresh leaves prior to liquid nitrogen exposure. Right: Homogenized leaves after grinding
Lanes 1 & 2: Rice leaf DNA. Lanes 3-5: λDNA standards. Lanes 6 & 7: Marker.
