Instrument: Minilys Personal Homogenizer
Sample: Transfected HEK 293T producer cells
Verses 4 freeze-thaw cycles method
Lysates from each method are used to transduce cultured HEK 293T and rAAV infectivity is compared by observation under the microscope.
The images show that the Minilys efficiently releases rAAV vectors on the resulting crude cell lysate is effectively used to transduce HEK 293T in image A. Transduction efficiency is similar compared to the freeze-thaw method in image B.