Instrument: Precellys Evolution
Sample: 9 ml of E. coli diluted 1/1000 (3,15x108 CFU/ml)
Precellys® Evolution: 8500 rpm, 3x30 sec, 30 sec break
Lysis in duplicate
Determination of the concentration: % mortality : [(Ci-Cgrinding)/Ci]x100
After 24h of incubation at 37°C, results were observed. The plate count is linear for E. coli over the range of 30 - 300 CFU on a standard sized Petri dish. We kept the two dilutions in this range to calculate the lysing efficiency
|1/VK05_15mL||126/130||19/13||1,3 x 104||96%|
Table1: results of the lysing step with 9 ml of bacterial suspension, in duplicate. Each lysing suspension was spread on an agar plate in duplicate (100 µl).
Concentration of the starting solution was validated by spreading 100 µl on agar plate of corresponding dilution.
The VK05 15ml kit is very efficient as we observed percentage of mortality > 95% after lysing in comparison with the initial suspension of bacteria
From Bertin Technologies