RNA extraction from Streptomyces (Actinobacteria)
Instrument: Precellys® 24
Sample: Streptomyces mycelium leaf
Protocol:
- Add 20mg of Streptomyces mycelium to tubes from the 2ml microorganism lysing kit.
- Run the Precellys at 6500 rpm, 2 x 23s with a 10s break.
- Extract RNA with the trizol method
Results:
Total extracted RNA was run on a gel and visualized by ethidium bromide staining and UV transillumination. Total RNA extracted from Streptomyces was almost twice the amount recovered with enzymatic lysis (Qiagen RNAeasy Mini Kit).
Left lane: Total RNA from homogenization in the Precellys® 24. Right lane: Total RNA extracted using Qiagen RNAeasy Mini Kit.
