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• Homogenization of Leek Leaf

Homogenization of Leek Leaf

Instrument: Bullet Blender

Sample:  Allium ampeloprasum leaves.

Protocol:

1. Cut leaf into long, thin slices of 200mg or less and place each slice into a microcentrifuge tube.
2. Add a volume of beads equal to the mass of tissue. NOTE: 100mg ≅ 100μ
3. Add 2 volumes of buffer to the tube for every mass of sample.
4. Close the microcentrifuge tubes and place them into the Bullet Blender®.
5. Set controls for SPEED 9 and TIME 4 minutes. Press Start.
6. After the run, remove tubes from the instrument.
7. Visually inspect samples. If homogenization is unsatisfactory, run for another two minutes at speed 10.
8. Proceed with your downstream application.

Results: