DNA Extraction from Baculovirus Granules
Instrument: Precellys® 24
Sample: Baculovirus Cydia pomonella granulovirus (CpGV)
Protocol:
- Add occlusion bodies of baculovirus granules to the 2 ml microorganism lysing kit.
- Add 100 µl of 0.1 mg/ml yeast RNA (to saturate the surface of the glass beads)
- Homogenize by running the Precellys at 6500 rpm for 45 seconds.
- Centrifuge at 4,000 rpm for one minute.
Results:
A probe was designed for a gene encoding a structural protein of CpGV. The gene was amplified by qPCR and assessed for the presence of an amplicon of the expected size (59 bp). The results of agarose gel electrophoresis are shown below.
Lanes 1 and 4 are ladders. Lanes 2 and 3 are PCR products from two template CpGV DNA samples.
