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• Homogenization of Uterine Tissue

Homogenization of Uterine Tissue

Instrument: Bullet Blender

Sample:  Uterine Tissue

Protocol:

1. Cut uterine tissue into appropriately sized pieces for analysis (10mg-300mg).
2. OPTIONAL: Wash tissue 3x with ~1mL PBS. Aspirate. NOTE: This step removes some external contaminants (blood, etc.).
3. Samples
   1. Samples 50mg or greater. Place the sample in Navy bead lysis kit tube.
   2. Samples less than 50mg. Place the sample in Green bead lysis kit tube.
   3. Alternate protocol step for bulk beads. Place sample in microcentrifuge tube and add beads to the tube. Use a volume of beads equal to the mass of tissue. NOTE: 100mg ≅ 100μ
4. Add 0.025mL to 0.6mL buffer (2 volumes of buffer for every volume of sample).
5. Close the microcentrifuge tubes.
6. Place tubes into the Bullet Blender®.
7. Set controls for SPEED 8 and TIME 5 minutes. Press Start.
8. After the run, remove tubes from the instrument.
9. Visually inspect samples. If homogenization is unsatisfactory, run for another three minutes at the SPEED 10.
10. Remove sample tubes from the Bullet Blender®, add the appropriate buffer and proceed with your downstream application.

Results: