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• Mouse Femur Homogenization in the Bullet Blender

Mouse Femur Homogenization in the Bullet Blender

Instrument: Bullet Blender STORM

Sample: Mouse (M. musculus) femur

Protocol:

1. Isolate 10 - 300 mg samples of mouse femur.
2. Load beads into tubes as follows:
1. If using kits, place the samples in navy bead kit tubes.
2. If using bulk beads, place the samples in microcentrifuge tubes and add approx. 100 µl of 0.9 - 2.0 mm stainless steel blend beads along with 6x 3.2 mm stainless steel balls.
3. Add 2 volumes of buffer for every volume of beads (200 to 600 µl).
4. Securely close the microcentrifuge tubes and place them into the Bullet Blender®.
5. Set the Bullet Blender to a speed of 10 and a run time of 5 minutes. Press start.
6. After the run, remove the tubes from the instrument.
7. Visually inspect samples, if homogenization is unsatisfactory, run for another five minutes at speed 10.

Results: