RNA extraction from Arabidopsis with Minilys
Instrument: Minilys homogenizer
Sample: Arabidopsis seedlings
Protocol:
- Precellys kit: 03961-1-009 (1.4&2.8 ceramic beads mix).
- Add Samples: 5 Arabidopsis seedlings (two weeks old).
- Add Buffer: 1mL TRI Reagent (MRC TR118).
- Run the Minilys at 5000 rpm, 20 sec or 5000 rpm, 40s.
- Snap-frozen in N2 or not.
- Triplicate by condition.
Results:
Total RNA was extracted directly from the homogenized samples using the TRI Reagent protocol before being checked for quality and quantity.
Mean concentration of total RNA from 4 extracted pool samples snap frozen before TRI Reagent addition or not and homogenization with Minilys at 5000 rpm 20s or 40s. The corresponding mean absorption ratio (260/280) are also plotted.
