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Homogenization of Peanut

Instrument: Bullet Blender

Sample:  Arachis hypogaea

Protocol:

1. Cut peanut into appropriately sized pieces for analysis (50mg-150mg) and place into microcentrifuge tube.
2. OPTIONAL: Wash peanut 3x with ~1mL PBS. Aspirate. NOTE: This step removes some external contaminants and debris.
3. Add a volume of beads equal to the mass of tissue. NOTE: 100mg ≅ 100μ
4. Close the microcentrifuge tubes.
5. Place tubes into the Bullet Blender®.
6. Set controls for SPEED 8 and TIME 4 minutes. Press Start.
7. After the run, remove tubes from the instrument.
8. Visually inspect samples. If homogenization is unsatisfactory, run for another three minutes at the SPEED 10.
9. Remove sample tubes from the Bullet Blender®, add the appropriate buffer and proceed with your downstream application.

Results: