LPO extraction from tumor and non-tumor rats tissues
Instrument: Precellys® 24 Dual with Cryolys cooling device to have a constant temperature of 4ô€?²C within the homogenization chamber using liquid nitrogen.
Sample: Tumors from intestine, liver, and heart of rats (frozen)
Protocol:
- Precellys kits: 03961-1-009 (CKmix).
- Sample: ~100 mg of frozen tumors, intestine, liver, and heart from rats treated with DHA + epirubicin and from control rats (palm oil, no chemotherapy) .
- Buffer: ice-cold distilled water.
- Run the Precellys at 6500 rpm, 3x20sec, 50sec break.
- Lipid hydroperoxides (LPO) were extracted and assayed with a lipid hydroperoxides assay kit according to the manufacturer's instructions (Kit no. 705003, Cayman Chemical Company, Ann Arbor, MI, USA).
Results:
The combo Precellys & Cryolys cooling option are suitable and reliable systems to homogenize a large range of rats tissues and tumors to investigate molecular and cellular mechanisms of action of lipids.
LPO level in tumor and non-tumor tissues at baseline (control rats) and in response to treatment with DHA and epirubicin.
