By Kim McDonald, 08 June 2024
Sonication uses ultrasonic frequencies of 20 kHz or greater to disrupt cell membranes and ensure complete cell lysis. While most homogenization techniques are capable of cell lysis and homogenization, sonicators are particularly efficient. Where sonication stands out from other methods is its ability to shear chromatin and DNA in small sample volumes. DNA released from lysed nuclei can create a viscous sample that interferes with sample preparation and gel loading. Sonication shears DNA, decreasing viscosity, homogenizing the sample, and releasing DNA-bound proteins, leading to greater overall Western blot sensitivity.
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By Doran Amos, 17 December 2023
In recent years, the production and consumption of cannabis-derived products for medicinal and health-enhancing purposes has grown rapidly across the US and worldwide. In this article, we detail the key factors to consider when purchasing a homogenizer and summarize the main advantages and disadvantages of the most suitable homogenizer types for cannabis applications.
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By Doran Amos, 16 September 2022
Heat build-up during homogenization can be a significant challenge, especially when working with temperature-sensitive samples. So how can you prevent heat damage to your sample during homogenization? In this article, we provide specific tips on how to deal with sample overheating in four popular types of homogenizer.
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By Aimee O'Driscoll, 08 July 2021
We examine the issue of trace amounts of titanium in samples and what you can do to limit risks.
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By Aimee O'Driscoll, 22 June 2021
Need to decide between a bead mill homogenizer and a high pressure homogenizer? We describe both technologies including their pros and cons and reveal which applications they're most suitable for.
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By Aimee O'Driscoll, 19 May 2021
We take a look at the pros and cons of ultrasonic homogenization and high pressure homogenization. We also offer a comparison of the two technologies and summarize which types of applications each is most suitable for.
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By Aimee O'Driscoll, 18 April 2021
We look at the main factors to consider when deciding which horn is right for your application and the different types of probes available.
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By Aimee O'Driscoll, 13 February 2021
Selecting the right probe is a crucial step in any rotor-stator homogenization application. We explain the options available and how to choose the right generator for your needs.
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By Aimee O'Driscoll, 20 December 2020
We compare the pros and cons of rotor-stator homogenization and high pressure homogenization. In addition, we provide a summary of their features and functionality and reveal the types of applications they are most suitable for.
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By Aimee O'Driscoll, 21 March 2020
We take a look at the pros and cons of rotor-stator homogenization and ultrasonic homogenization. In addition, we provide a summary of which types of applications each technology is most suitable for.
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By Aimee O'Driscoll, 28 December 2019
We take a look at the pros and cons of bead mill homogenization and ultrasonic homogenization. In addition, we provide a summary of which types of applications each technology is most suitable for.
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By Aimee O'Driscoll, 31 October 2019
We take a look at the pros and cons of rotor-stator homogenization and bead mill homogenization. In addition, we provide a summary of which types of applications each technology is most suitable for.
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By Aimee O'Driscoll, 16 August 2019
Most types of homogenization processes involve a combination of multiple forces, including shear and impact. We explain what these forces are and in which types of homogenization they are most prominent.
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By Aimee O'Driscoll, 20 April 2019
We explain why ultrasonic homogenizers, as well as other types of ultrasonic equipment, such as ultrasonic baths, typically operate at a single frequency.
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By Aimee O'Driscoll, 05 March 2019
We discuss some of the issues you might observe when scaling up a rotor-stator homogenization process and the main factors to consider to make your scaleup as successful as possible.
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