Should You Sonicate Your Western Blot Lysates?

By Kim McDonald, 08 June 2024

Sonication uses ultrasonic frequencies of 20 kHz or greater to disrupt cell membranes and ensure complete cell lysis. While most homogenization techniques are capable of cell lysis and homogenization, sonicators are particularly efficient. Where sonication stands out from other methods is its ability to shear chromatin and DNA in small sample volumes. DNA released from lysed nuclei can create a viscous sample that interferes with sample preparation and gel loading. Sonication shears DNA, decreasing viscosity, homogenizing the sample, and releasing DNA-bound proteins, leading to greater overall Western blot sensitivity. 

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Ultimate Guide to Homogenizer Selection for Cannabis Applications

By Doran Amos, 17 December 2023

In recent years, the production and consumption of cannabis-derived products for medicinal and health-enhancing purposes has grown rapidly across the US and worldwide. In this article, we detail the key factors to consider when purchasing a homogenizer and summarize the main advantages and disadvantages of the most suitable homogenizer types for cannabis applications.

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How to prevent heat build-up during homogenization

By Doran Amos, 16 September 2022

Heat build-up during homogenization can be a significant challenge, especially when working with temperature-sensitive samples. So how can you prevent heat damage to your sample during homogenization? In this article, we provide specific tips on how to deal with sample overheating in four popular types of homogenizer.

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How to Choose a Homogenizer Probe

By Aimee O'Driscoll, 13 February 2021

Selecting the right probe is a crucial step in any rotor-stator homogenization application. We explain the options available and how to choose the right generator for your needs.

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Rotor Stators Versus High Pressure Homogenizers

By Aimee O'Driscoll, 20 December 2020

We compare the pros and cons of rotor-stator homogenization and high pressure homogenization. In addition, we provide a summary of their features and functionality and reveal the types of applications they are most suitable for.

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The Forces Involved in Homogenization Explained

By Aimee O'Driscoll, 16 August 2019

Most types of homogenization processes involve a combination of multiple forces, including shear and impact. We explain what these forces are and in which types of homogenization they are most prominent.

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