The MicroMincer is a compact, hand-operated screw press which macerates 0.3 grams or less of soft* fresh plant or animal tissue (e.g. muscle, liver, brain, epicotyl and pulpy leaf tissue) without sample loss, heat generation, foaming or aerosol problems. Much of the sample, without added buffer, is pushed through a 250 micron stainless-steel sieve under thousands of pounds force. In the case of plant tissues, fibrous, cellulosic pulp is left behind while cell juice and intracellular products are extruded. The resultant extract can be deposited directly in microtubes.
The Micromincer eliminates re-concentration of sample extract, as no additional buffer is required for use.
The stainless steel MicroMincer utilizes disposable polypropylene liners. Thus, multiple samples can be quickly processed without a need to clean or risk of contamination between samples.
The isolation of single cells from tissue samples commonly involves enzymatic digestion of the extracellular connective matrix. The first step in the process is to increase access of added digestive enzymes to the tissue by chopping the tissue into pieces with a scissors or sharp blades. The MicroMincer offers a significant improvement to this process, yielding much smaller tissue pieces of tissue. This greatly speeds up the rate of enzymatic disaggregation and maximizes the yield of undamaged, viable cells. The MicroMincer reduces the tissue to pieces so small that diffusion of nutrients and gases are not limiting during the prolonged incubation with digestive enzymes.
*Highly fibrous animal tissue containing collagen (e.g., skin, some tumors, cartilage) cannot pass through the screen. Likewise, highly fibrous plant tissue cannot be processed.
|Homogenizer Type||Mortar & Pestle|
|Sample Throughput||1 sample|
|Sample Volume (min)||100 µl|
|Sample Volume (max)||500 µl|
|Usage / Placement||Handheld|