|Standard Warranty (2-year)||NA:BB50-AU||
|Extended Warranty (4-year)||NA:BB50-AU+EW-GOLD||
The Bullet Blender® 50 Gold enables you to homogenize, disrupt, or lyse up to 8 large tissue or cell culture samples (up to 3 mg solid weight, 10-15 ml total volume) at a time in standard polypropylene tubes, all while keeping them at refrigerated temperatures near 4°C.. Load the samples into the tubes, place them in the Bullet Blender, set the time and speed and you're ready! The "bullets" in the instrument vigorously strike all of the tubes and homogenize your sample in minutes.
The "Gold" models of the Bullet Blenders include a number of upgrades. The Bullet Blender Gold includes dry ice cooling to keep samples at or near 4°C, have built-in sound insulation for quieter operation, and an upgraded motor for more efficient homogenization of tougher samples as well as faster homogenization of soft ones! Fill the side compartment with dry ice and cold air will circulate through the inside of the instrument. The 4°C signal will light up when the temperature is reached. The air circulation will adjust according to homogenization conditions, allowing the contents of the tube to reach and maintain a temperature of 4°C.
This "deluxe" size Bullet Blender Gold homogenizes up to 8 large samples (up to 3.5 g) at a time in standard 50 ml skirted polypropylene centrifuge tubes. There is also a standard model which homogenizes up to 24 small samples (up to 300 mg) at a time in standard 1.5 ml polypropylene snap-cap microcentrifuge tubes or the mid-sized "5" model which homogenizes up to 12 mid-sized samples (up to 1g) at a time in 5 mL Eppendorf® snap-cap tubes.
All of the samples are automatically homogenized in the same manner to minimize sample-to-sample variability.
There are no probes or blades to cause cross contamination or sample loss, which also means there are no parts to clean between runs. The sample tubes are kept closed and self-contained during homogenization.
The Bullet Blender Gold uses dry ice for easy, inexpensive, maintenance-free cooling that keeps samples at 4°C during processing. The instrument uses little power due to the unique, patented design, so there is very little heat build up in the instrument or inside the sample tubes. It uses a DC motor to agitate the individual tubes. The Bullet Blender Gold is designed for reliability and durability, with few moving parts and a very low rate of motor wear.
Simply place your sample, beads and buffer in standard tubes, and load the tubes into the Bullet Blender. Tune the extent of disruption by adjusting the run time and speed. Afterwards, place the sample tubes in a centrifuge to separate the lysate. No special holders are needed. There are no clamp plates nor locking rings to fiddle with.
All Bullet Blenders include a 30 day money back guarantee and a 2 year warranty.
from Plant Samples
from Animal Samples
from Microbial Samples
Genomic DNA samples in agarose gel stained with ethidium bromide.
Lanes 1-4 = Tobacco leaf DNA
λ = phage λ Hind II digest
Lanes 5-8 = Rice leaf DNA
Total RNA gel from mouse tail snips.
RNA integrity number = 8.5
Data courtesy of Madhu Prasad @ U. Michigan
E. coli sample.
SDS Page gel stained with coomassie blue.
wc = whole cell. sf = soluble fraction.
Left two lanes control. Right two lanes induced.
|Homogenizer Type||Bead Mill|
|Sample Throughput||8 samples|
|Sample Volume (min)||100 µl|
|Sample Volume (max)||10 ml|
|Usage / Placement||Benchtop|
Is there any added benefit to using the Bullet Blender 50 Gold in a cold room?
In our opinion that's probably overkill, but it can't hurt and may improve temperature stability slightly.
The Bullet Blender Gold detects the air temperature inside the instrument where the samples are located and augments the fan speed which pulls air through the dry ice chamber to ensure samples stay as close to 4°C as possible. By both pulling air that is already cool then applying additional cooling to it as necessary, there is a greater certainty that samples will remain cold through the entire homogenization. Because the temperature sensor will cut the fan if the temperature drops below 4°C, there is no risk of the samples freezing.
What is the RPM range of the Bullet Blender 50 Gold?
Next Advance does not disclose the RPM specifications for the Bullet Blenders. The reason they do this is because the mechanism of action for the Bullet Blenders (striking) is very different from the mechanism of action for other laboratory bead mill homogenizers, which almost exclusively act via shaking. Direct comparisons with different bead mills from different manufacturers based on RPMs, including using protocols from other instruments based on RPM, are not possible.
The best we can tell you is that the speed setting "1" is a speed which would be good for a moderately vigorous mixing, and the maximum speed is the most power you will get from the instrument. The specific RPMs which correspond to the numbers on the dial are not documented by Next Advance.
If you have questions pertaining to the processing power of the Bullet Blender 50 Gold, please contact us for more information.Next Advance 2013-06-09