Instrument: Precellys® 24 Dual
Sample: Mycobacteria obtained from cultures (note that pathogenic mycobacteria must be processed under appropriate biosafety containment).
Protocol:
Results:
Mechanical disruption of the mycobacterial cell wall, after incubation with 4% SDS (final concentration), was found to be crucial for sufficient and satisfactory yields.
DNA yield achieved when testing both combinations of DNA extraction procedures (chemical lysis only vs chemical and mechanical disruption)