Instrument: Precellys® 24
Sample: Atlantic salmon (Salmo Salar) muscle
*In this instance, the lab was looking to measure cathepsin B. You may substitute a more appropriate buffer for your application, if necessary.
Salmon muscle tissue was fully and thoroughly homogenized by the Precellys 24, allowing downstream fluorometric detection of Cathepsin activity from the prepared muscle homogenate supernatants.
a) Frozen salmon muscle. b) Muscle prepared for homogenization. c) Muscle post-homogenization. d) Muscle homogenates after centrifugation.
Total cathepsin activity in muscle homogenates from salmon slaughtered without crowding stress (NS), short-term crowding stress (SS) or long-term crowding stress (LS).