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RNA Extractions of Microorganisms

Instrument: Precellys® 24 Dual

Sample:  Escherichia coli & Bacillus globigii spores

Protocol:

  • Precellys kits: CK28 (big ceramic beads) and VK05 (small glass beads)
  • Sample : Escherichia coli bacteria/ Bacillus globigii spores in buffer.
  • Run the Precellys®24 at 6500rpm, 2x23 sec.

Results:

Results were conducted in collaboration with LGN (Laboratory of Molecular Genetic of the

Neurotransmission). E.coli and spores of B.globigii were lysed with Precellys kit compared to standard procedures. RNA extraction was performed from the 2 microorganisms following each sample preparation.

The Precellys®24 offers a sample preparation step more efficient on difficult micro-organisms than traditional procedures. The RNA was not denatured. The Precellys®24 was also well appreciated as a cross contamination free system and for the time saved on sample preparation.

 
  1. E.coli (Precellys)
  2. E.coli (standard)
  3. B.globigii spores (Precellys)
  4. B.globigii spores (Standard)