Instrument: Precellys® 24 Dual
Sample: Human skin biopsies
Compared to classical lysis (chaotropics ions + ß- Mercapto ethanol + Vortex), RNA yield increased from 2 to 4 fold (Nanodrop quatification). RNA quality was assessed using a 2100 Bioanalyser. RNA Integrity Number (RIN) is over 8, meaning good quality.
RNA extracted in highest quantity using Precellys®24 allowed us to study transcription modifications of several genes implicated in pigmentation or inflammation after UV-solarspectrum-radiation corresponding to 1 to 2 MED (Minimal Erythema Dose).