RNA Extraction from Human (Homo Sapiens) Colon

Sample: Human (H. Sapiens) colon

Protocol:

Add 30 mg of human colon to the tubes from the 2 ml hard tissue homogenizing kit.
Add 250 µl Trizol.
Run the Precellys 24 at 6500rpm for 2 x 10s with a 10s break followed by 1 x 15s.

Results:

RNA yields were compared against two competitors. The Precellys provided a substantially higher RNA yield than than one competing instrument while providing a similar yield to the other. RNA quality and purity were high.

RNA yield for homogenized human colon by the Precellys 24 and two competitors.

RNA yield and quality for homogenized human colon by the Precellys 24 and two competitors.