Instrument: Precellys® 24
Sample: Nicotiana benthamiana leaf
*You may substitute another buffer if your downstream application is different.
Proteins were analyzed by SDS-PAGE and western blot. Three GFP-fusion proteins are measured in the gel image below.
Western-blot analysis of protein extracts from N. benthamiana leaf cells transiently expressing CLV1-GFP, CLV2-GFP, or CRN-GFP. An anti-GFP antibody was used for detection. The Ponceau stained protein bands of Rubisco are shown as a loading control. WT = wild type.