Instrument: Minilys homogenizer
Sample: Mouse spleen, colon, lung
The protein content of the samples was estimated with the QuiantiT Protein assay kit Invitrogen Q33211, using a 100-fold dilution of the original sample. Excellent results were obtained from different mice tissues using the Minilys apparatus for tissue lysis.
Protein recovery in 100μl buffer volume compared to the initial weight of the lysed spleen and colon tissue obtained with Minilys.