Instrument: Precellys® 24 Dual
Sample: Culture pellets from Ostreococcus tauri, Thalassiosira pseudonana, Synechococcus sp. WH7803, Prochlorococcus marinus PCC 9511 and Synechococcus sp. WH8101 re-suspended in buffer.
Results: Total protein quantification, Immunoblotting with relevant antibodies. Sample throughput and reproducibility are higher than with sonication/flash freeze protocol
Comparison of ribulose-1,5-bisphosphate carboxylase oxygenase (RbcL) immunodetection from phytoplankton after protein extraction through sonication or Precellys bead beating. RbcL was detected by immunoblotting using a chicken anti-RbcL primary antibody and a horseradish peroxidase-conjugated goat anti-chicken secondary antibody.