Sample : 50 – 150 mg of brain and lymphoid tissues
Buffer : glucose 5% (500 - 1500μl) added after grinding
Run the Precellys®24 for Brain tissues at 6500rpm, 2x30 sec.,15 sec. break. For Lymphoid tissues at 6500rpm, 3x30 sec., 20 sec. break
Prion protein extraction
ELISA analysis and Western Blot
After prion protein extraction, the pathologic protein is detected and quantified by using specific antibodies through an ELISA test, and the pattern of the glycozylated protein is analyzed by Western Blot.