Protein extraction from mouse heart using the Minilys
Instrument: Minilys homogenizer
Sample: Mouse heart
- Add 920 mg of mouse heart to 2 ml tissue homogenizing kits (CKMix / BER:KT03961-1-009.2).
- Add 920 µL of M-PER buffer
- Run at 5000 RPM for 4 cycles of 20 seconds each with a 5 second break between cycles.