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Protein extraction from mouse heart using the Minilys



Instrument: Minilys homogenizer

Sample: Mouse heart

Protocol:

  • Add 920 mg of mouse heart to 2 ml tissue homogenizing kits (CKMix / BER:KT03961-1-009.2).
  • Add 920 µL of M-PER buffer
  • Run at 5000 RPM for 4 cycles of 20 seconds each with a 5 second break between cycles.