Mouse Femur Homogenization in the Bullet Blender

Instrument: Bullet Blender STORM

Sample: Mouse (M. musculus) femur


  1. Isolate 10 - 300 mg samples of mouse femur.
  2. Load beads into tubes as follows:
    1. If using kits, place the samples in navy bead kit tubes.
    2. If using bulk beads, place the samples in microcentrifuge tubes and add approx. 100 µl of 0.9 - 2.0 mm stainless steel blend beads along with 6x 3.2 mm stainless steel balls.
  3. Add 2 volumes of buffer for every volume of beads (200 to 600 µl).
  4. Securely close the microcentrifuge tubes and place them into the Bullet Blender®.
  5. Set the Bullet Blender to a speed of 10 and a run time of 5 minutes. Press start.
  6. After the run, remove the tubes from the instrument.
  7. Visually inspect samples, if homogenization is unsatisfactory, run for another five minutes at speed 10.


Before and after of mouse femur homogenization in the Bullet Blender STORM