Metabolomic study from clam gill

Instrument: Precellys® 24 Dual

Sample: Clam gill tissue


  • Precellys® kit: 03961-1-003 (1.4mm ceramic beads).
  • Add ~100 mg (wet mass) of gill tissue.
  • Add Extraction solvents: 4 ml/g (v/w) methanol and 0.85 ml (v/w) water.
  • Run the Precellys at 6400 rpm, 2x10 sec, 30 sec break.


Metabolomics analysis: Extraction with methanol, chloroform and water to extract the polar metabolites, storage at -80°C, one dimensional 1H NMR spectroscopy (500 M), data analysis.