Instrument: Precellys® 24
Sample: Spleen and Skin
Following tissue homogenization, the number of viable bacterial cells (CFU/ml) present was determined by serial dilution in sterile 0.7% saline and plating onto nutrient agar. Results indicate that for all treatment groups the type of bead and the processing time had no significant effect on the viability of S. pyogenes (t test p> 0.1)
The effect of tissue homogenization on the viability of S. pyogenes. Data represents the mean CFU/ml from triplicate samples with error bars indicating the standard derivation. The inoculum represents the CFU/ml added to each sample.