Isolation of RNA from Rat Liver

Instrument: Precellys® 24 Dual

Sample:  Mouse murine pancreas (organs in which the virus replicates)


  • Precellys kits: CK28 (big ceramic beads)
  • Sample : 30 mg of rat liver previously stored in RNAlater (Ambion) at -20°C
  • Buffer : 600μl RTL buffer (RNeasy Mini Kit,Qiagen)
  • Run the Precellys®24 at 5000rpm, 2x10 sec., 10 sec. break



These results were concluded with the collaboration of Altana Pharma AG. Analysis of the subsequently isolated RNA using the Agilent 2100 bioanalyzer.


The Precellys®24 is an ideal tool for the lysis of rat liver for subsequently isolating RNA. Particularly for higher numbers the Precellys® is superior to the formerly used mechanical blender, regarding time consumption and reproducibility.