Instrument: Precellys® 24 Dual
Sample: Heart, liver, spleen
RNA integrity and yield was evaluated using an Agilent Bioanalyzer, and an average RIN (RNA integrity number) value of 9 (highest score is 10) was observed using the Precellys, with RNA concentration falling between 400-1800 ng/ul. The gel displays clean 18S and 28S rRNA bands with little or no degradation (Figure 1A). RIN values of “N/A” were observed after pulverization, which is indicative of highly degraded RNA as observed on the gel (Figure 1B).
The Precellys24-Dual significantly improves RNA integrity and yield compared to our previous pulverization method. In addition, the time spent on sample homogenization was decreased from one hour to 30 seconds