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Homogenization of Sugar Cane

Instrument: Bullet Blender

Sample:  Sugar cane center pulp

Protocol:

1. Place up to 300mg of sugar cane pulp into microcentrifuge tubes.
2. Add a volume of beads equal to the mass of the sample.
3. Add 2 volumes of buffer to the tube for every mass of sample (ex. for 100 mg sugar cane, add 200mL buffer)
4. Close the microcentrifuge tubes and place them into the Bullet Blender®.
5. Set controls for SPEED 9 and TIME 3 minutes. Press Start.
6. After the run, remove tubes from the instrument.
7. Visually inspect samples. If homogenization is unsatisfactory, run for another two minutes at speed 10.
8. Proceed with your downstream application.

Results: