Set controls for SPEED 10 and TIME 4 minutes. Press Start.
After the run, remove tubes from the instrument.
Visual inspection of the homogenate is difficult due to the formation of emulsions with the oils from the plant tissue in aqueous buffers. To verify sufficient homogenization, you may place forceps or a spatula inside the tube to search for remaining large fragments. If homogenization is unsatisfactory, run for another two minutes at the SPEED 10.
Remove sample tubes from the Bullet Blender® and proceed with your downstream application.