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Homogenization of Drosophila for lipid analysis

Instrument: Precellys® 24

Sample: Drosophila L3 larvae or adult heads.

Protocol:

  • Precellys® lysing kit: 03961-1-002 (ceramic beads 2.8mm)
  • Add Drosophila L3 larvae (15) or Drosophila adult heads (80).
  • Buffer: Water.
  • Run the Precellys at 5500 rpm, 2x25 sec, 10 sec break.
  • Centrifugation steps: 5000 rpm - 60s.

Results:

Analysis of lipid content: Samples were dilapidated according to Bligh and Dyer, 1959 for thin layer chromatography studies and gas chromatography coupled with mass spectrometry (GC/MS) was carried out after FA methyl ester derivatization according to Ecker et al., 2010

GC/MS analysis of fatty acids from Drosophila L3 larvae (Myristic acid (C14:0), palmitic acid (C16:0), palmitoleic acid (C16:1), oleic acid (C18:1) and linoleic acid (C18:2)).