Homogenization of Drosophila Adults

Instrument: Bullet Blender or Bullet Blender Blue

Sample: Fruit fly (D. melanogaster) adults


  1. Place 10 - 300 mg of Drosophila into microcentrifuge.
  2. Add a volume of 0.5mm zirconium oxide or 0.5 mm zirconium silicate beads equal to the mass of tissue.  A useful guideline is 100mg ≅ 100μL, as the flies may lie loose in the tube and occupy more volume than they would compressed.
  3. Add 2 volumes of buffer for every volume of sample.
  4. Securely close the microcentrifuge tubes and place them into the Bullet Blender®.
  5. Set the Bullet Blender to a speed of 8 and a run time of 3 minutes. Press start.
  6. After the run, remove the tubes from the instrument.
  7. Visually inspect samples, if homogenization is unsatisfactory, run for another two minutes at speed 9.


Drosophila homogenized in the Bullet Blender