Enzyme assay from frozen abdominal krill tissue

Instrument: Precellys® 24 with Cryolys cooling device to have a constant temperature of 4°C within the homogenization chamber using liquid nitrogen.

Sample:  Abdominal Krill Tissue (frozen)


  • Precellys kits: 013961-1-009 (CKmix).
  • Sample: ~100 mg (fresh weight) of frozen abdominal krill tissue.
  • Extraction solvents: ice-cold deionized water at a concentration of 100 mg fresh weight (fw) mL–1.
  • Run the Precellys 24 at 5000 rpm, 2x15sec, 10s break.
  • Enzyme assay: Centrifugation of homogenate, spectrophotometrically analysis of supernatant in a specific buffer, data analysis.


Time course experiments require a standardized and equably homogenization of biological tissues that guarantee a reliable temporal analysis of data. In particular, enzyme assays require a low temperature during the homogenization process to maintain enzyme activity.

During this study, the Cryolys guaranteed a constant temperature of +4°C within the homogenization chamber and thus avoided an uncontrolled defrosting of the frozen samples.

Oscillatory rhythms of the metabolic key enzyme citrate synthase (CS) in light-dark (LD) cycle and in constant darkness (DD).