Laboratory Supply Network is open and operational during the COVID-19 pandemic. You can find updates pertaining to any supply chain issues in this post.

Drug extraction from whole iris-ciliary body pork

Instrument: Minilys homogenizer vs  Precellys® 24 Dual vs Probe sonicator

Sample:  Whole iris-ciliary body from pork

Protocol:

  • Precellys kits: ceramic beads 7mL vial (réf.0904-01) +2 beads (Réf.03961-1-106).
  • Sample: Thawed whole iris-ciliary body pork (~0.7 g) into 7mL vial.
  • Buffer: 2 volumes of sodium chloride solution.
  • Run the Minilys & Precellys at 5000 rpm, 4x30sec (15sec break).

Results:

Analysis was performed after liquid-liquid extraction; LC-MS/MS system. The analyte levels are equivalent. No difference involving the homogenizer is detectable.