Instrument: Minilys homogenizer vs Precellys® 24 Dual vs Probe sonicator
Sample: Whole rat brain
Analysis was performed after liquid-liquid extraction; LC-MS/MS system. The analyte levels by samples are equivalent. No difference involving the three homogenizing techniques are detectable. Despite one sample prep in 7mL vial, Minilys is preferred rather the probe sonicator method which is fastidious and time consuming with cleaning steps and potential contamination between samples.