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DNA extraction from liver tissue with Minilys

Instrument: Minilys homogenizer

Sample: Liver tissue

Protocol:

  • Precellys kit: 03961-1-010 (1.4 & 2.8 ceramic beads mix).
  • peqGOLD Tissue DNA mini Kit
  • Add 30 mg of liver tissue
  • Add 100 μl TE buffer
  • Run the Minilys at 5000 rpm for 15 sec

Results:

Total genomic DNA was extracted directly from the homogenized samples using peqGOLD Tissue DNA mini Kit before being checked for quality and quantity using NanoDrop analysis.

 

Mean concentration of total genomic DNA from 3 extracted samples of liver tissue with Minilys (run 1 and 2) in comparison with Precellys. The corresponding mean absorption ratios (260/280; 260/230) are also plotted.