Instrument: Precellys® 24 Dual
Sample: C.elegans with S. Typhimurium strain L1019.
Infection load was assessed through viable counts for 5 days / each 24 hours. lys-7 and abl-1 mutant animals are tolerant of S. Typhimurium infection. There was no difference between any of the strains (p.0.2) showing that lys-7 and abl-1 single mutants are tolerant to S. Typhimurium infection for at least six days.
Lysing nematodes infected with Salmonella using the Precellys does not affect the bacteria viability, allowing infection load to be accurately assessed.