Bullet Blender® 5 Gold

Category Bead Mill Homogenizer
Manufacturer Next Advance
Country of manufacture the United States
Overview

The Bullet Blender Gold enables you to homogenize, disrupt, or lyse up to 12 tissue or cell culture samples of up to one gram simultaneously in standard polypropylene tubes. Load the samples into the tubes, place them in the Bullet Blender, set the time and speed and you're ready! The "bullets" in the instrument vigorously strike all of the tubes and homogenize your sample in minutes.

The "Gold" models of the Bullet Blenders include a number of upgrades.  The Bullet Blender Gold includes dry ice cooling to keep samples at or near 4°C, have built-in sound insulation for quieter operation, and an upgraded motor for more efficient homogenization of tougher samples as well as faster homogenization of soft ones!  Fill the side compartment with dry ice and cold air will circulate through the inside of the instrument.  The 4°C signal will light up when the temperature is reached. The air circulation will adjust according to homogenization conditions, allowing the contents of the tube to reach and maintain a temperature of 4°C.

The Bullet Blender Gold comes in three sizes:

  • The standard Bullet Blender Gold homogenizes up to 24 small samples (up to 300 mg) at a time in standard 1.5 ml polypropylene snap-cap microcentrifuge tubes.
  • The Bullet Blender 5 Gold homogenizes up to 12 mid-sized samples (up to 1g) at a time in 5 mL Eppendorf® snap-cap tubes.
  • The deluxe Bullet Blender 50 Gold homogenizes up to 8 large samples (up to 3.5 g) at a time in standard 50 ml skirted polypropylene centrifuge tubes.

Consistent Results

All of the samples are automatically homogenized in the same manner to minimize sample-to-sample variability. 

No Cross-Contamination or Sample Loss

There are no probes or blades to cause cross contamination or sample loss, which also means there are no parts to clean between runs. The sample tubes are kept closed and self-contained during homogenization. 

Samples Stay Cool

The Bullet Blender Gold uses dry ice for easy, inexpensive, maintenance-free cooling that keeps samples at 4°C during processing.  The instrument uses little power due to the unique, patented design, so there is very little heat build up in the instrument or inside the sample tubes. It uses a DC motor to agitate the individual tubes.  The Bullet Blender Gold is designed for reliability and durability, with few moving parts and a very low rate of motor wear. 

Easy To Use

Simply place your sample, beads and buffer in standard tubes, and load the tubes into the Bullet Blender. Tune the extent of disruption by adjusting the run time and speed. Afterwards, place the sample tubes in a centrifuge to separate the lysate. No special holders are needed. There are no clamp plates nor locking rings to fiddle with. 

Risk Free Purchase

All Bullet Blenders include a 30 day money back guarantee and a 2 year warranty.

 

 DNA RNA Protein

from Plant Samples

from Animal Samples

from Microbial Samples

 

 Genomic DNA samples in agarose gel stained with ethidium bromide.

Lanes 1-4 = Tobacco leaf DNA

λ = phage λ Hind II digest

Lanes 5-8 = Rice leaf DNA

Total RNA gel from mouse tail snips.

RNA integrity number = 8.5

Data courtesy of  Madhu Prasad @ U. Michigan

E. coli sample.

SDS Page gel stained with coomassie blue.

wc = whole cell.  sf = soluble fraction.

Left two lanes control. Right two lanes induced.

Key Attributes
Homogenizer Type Bead Mill
Sample Throughput 12 samples
Sample Volume (min) 100 µl
Sample Volume (max) 3 ml
Usage / Placement Benchtop
Cooling Yes
Warranty 2 years
Noise Level 90dB
Weight 30 lb
Width 18"
Depth 14"
Height 15"
FAQ

Is there any added benefit to using the Bullet Blender 5 Gold in a cold room?

In our opinion that's probably overkill, but it can't hurt and may improve temperature stability slightly.

The Bullet Blender Gold detects the air temperature inside the instrument where the samples are located and augments the fan speed which pulls air through the dry ice chamber to ensure samples stay as close to 4°C as possible. By both pulling air that is already cool then applying additional cooling to it as necessary, there is a greater certainty that samples will remain cold through the entire homogenization. Because the temperature sensor will cut the fan if the temperature drops below 4°C, there is no risk of the samples freezing.

What is the RPM range of the Bullet Blender 5 Gold?

Next Advance does not disclose the RPM specifications for the Bullet Blenders. The reason they do this is because the mechanism of action for the Bullet Blenders (striking) is very different from the mechanism of action for other laboratory bead mill homogenizers, which almost exclusively act via shaking. Direct comparisons with different bead mills from different manufacturers based on RPMs, including using protocols from other instruments based on RPM, are not possible.

The best we can tell you is that the speed setting "1" is a speed which would be good for a moderately vigorous mixing, and the maximum speed is the most power you will get from the instrument. The specific RPMs which correspond to the numbers on the dial are not documented by Next Advance.

If you have questions pertaining to the processing power of the Bullet Blender 5 Gold, please contact us for more information.

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