Instrument: Minilys homogenizer
Instrument: Precellys® 24 Dual
Sample: Torafugu pufferfish (Takifugu rubripes) liver or muscle
Analysis performed on denatured agarose gel (0.9%) electrophoresis stained with Sybr Green. The amount of RNA was determined using a spectrophotometer. The quality of extracted RNA from torafugu pufferfish was good. For the torafugu pufferfish muscle, which is exceptionally hard among the fish muscle, 5000 rpm 2x 30sec gave very good RNA yield. The 4000 rpm treatment was not enough (the homogenate looked somewhat granular). The RNA concentration extracted from the fish liver using Minilys was somewhat smaller than the amount of RNA extracted using Precellys24.