RNA extraction from Cyanobacteria using the Precellys 24

Instrument: Precellys® 24

Sample: Cyanobacteria (Nostoc punctiforme)


  • Add cyanobacteria pellet to the 2 ml tough microorganism lysing kit.
  • Add PGTX buffer*.
    • PGTX buffer formula: phenol (39.6 g), glycerol (6.9 mL), 8-hydroxyquinoline (0.1 g), EDTA (0.58 g), sodium acetate (0.8 g), guanidine thiocyanate (9.5 g), guanidine hydrochloride (4.6 g) and Triton X-100 (2 mL).  Final pH should be approximately 4.2.  The PGTX extraction mixture should form a monophasic solution at room temperature.
  • Run the precellys at 6500rpm for 2 x 20 seconds with a 10s break.
  • Centrifuge the homogenate to remove any residual solids


RNA yield and purity were assessed using a NanoDrop ND-1000, an automated gel electrophoresis system, and RT-PCR.  The Precellys 24 provided improved RNA yields and RNA purity compared to the user's previous method (high-temperature 95°C cell disruption in PGTX buffer).  Use of PGTX buffer improved RNA purity and decreased experimental cost compared to Trizol.
Cyanobacteria RNA quality and yield after extraction using a Precellys 24
 Extracted RNA yield and purity using Precellys with PGTX (left), Precellys with Trizol (center), and high-temperature lysis with PGTX (right).
RNA electrophoresis gel from Cyanobacteria lysed using a Precellys
Gel image of RNA obtained from cyanobacteria N. punctiforme using the Precellys with PGTX ("PGTX beads"), the Precellys with Trizol ("Trizol beads") or high-temperature lysis with PGTX ("PGTX 95").