RNA Extraction from Caenorhabditis

Instrument: Precellys® 24 Dual

Sample: C.elegans from a full 9cm petri dish (either mixed stage, or staged, depending on requirements)


  • Precellys® lysing kit: 03961-1-004 (0.5mm glass beads)
  • Add sample: Suspension of C.elegans gently pelleted by centrifugation (1500rpm for 1min) and washed three times with M9 buffer before resuspending in 500μl of M9 buffer.
  • Run the Precellys at 6400 rpm, 2x10 sec, repeat for a total time of 40secs.


RNA was isolated directly from the extract using Qiagen RNeasy kit before being checked for quality and quantity using NanoDrop analysis.

On average a yield of 1.5 – 2 μg/μl of RNA was obtained per 9cm petri plate. This RNA was then used for RT-PCR to quantify gene expression studies as shown