Instrument: Precellys® 24 Dual
Sample: C.elegans from a full 9cm petri dish (either mixed stage, or staged, depending on requirements)
RNA was isolated directly from the extract using Qiagen RNeasy kit before being checked for quality and quantity using NanoDrop analysis.
On average a yield of 1.5 – 2 μg/μl of RNA was obtained per 9cm petri plate. This RNA was then used for RT-PCR to quantify gene expression studies as shown